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Tuesday, July 14, 2020 | History

3 edition of Quantitative paper chromatography of ¹⁴C-labelled long-chain fatty acids found in the catalog.

Quantitative paper chromatography of ¹⁴C-labelled long-chain fatty acids

Heinz J. M. Hansen

Quantitative paper chromatography of ¹⁴C-labelled long-chain fatty acids

by Heinz J. M. Hansen

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  • 24 Currently reading

Published by (Gjellerups Boghandel) in Copenhagen .
Written in English

    Subjects:
  • Fatty acids -- Analysis.,
  • Paper chromatography.,
  • Carbon -- Isotopes.

  • Edition Notes

    Statementby Heinz J. M. Hansen.
    SeriesRisö report ;, 210
    Classifications
    LC ClassificationsQC770 .D4 no. 210
    The Physical Object
    Pagination59 p.
    Number of Pages59
    ID Numbers
    Open LibraryOL4059851M
    LC Control Number79533852

    Summary. Two methods are described, both currently used in our laboratory, for the quantitative analysis of very long-chain fatty acids, phytanic acid and pristanic acid in plasma and cultured fibroblasts by gas-liquid chromatography.   Conversion of free fatty acids into FAMEs A mg amount of a sample of anhydrous free fatty acids (FFAs) (or the sodium salt) is weighed into an 8-ml Sovirel culture tube with a screw-cap (PTFE liner) and 6 ml of heptane are added.

    Method development for the quantitative determination of short chain fatty acids in microbial samples by solid phase extraction and gas chromatography with flame ionization detection Hayoung Kim1, Joseph Kwon2, So Young Choi3 and Yun Gyong Ahn1* Abstract. quantitative measurement of fish oils fatty acids were carried out by gas chromatography coupled with mass spectrometry and by 1H-NMR spectroscopy. Fourteen individual fatty acids were identified using GC-MS technique. GC-MS was applied on fatty acids methyl esters. Identification of fatty acids .

      We present a liquid chromatography – mass spectrometry (LC-MS) method for long-chain and very-long-chain fatty acid analysis, and its application to 13 C-tracer studies of fatty acid metabolism. Fatty acids containing 14 to 36 carbon atoms are separated by C 8 reversed-phase chromatography using a water-methanol gradient with tributylamine as ion pairing agent, ionized by . Long-chain acyl-CoA esters (LCACoAs) are activated lipid species that represent key substrates in lipid metabolism. The relationship between lipid metabolism disorders and type 2 diabetes has attracted much attention to this class of metabolites. This paper presents a highly sensitive and robust on-line LC/MS2 procedure for quantitative determination of LCACoAs from rat liver.


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Quantitative paper chromatography of ¹⁴C-labelled long-chain fatty acids by Heinz J. M. Hansen Download PDF EPUB FB2

See more details, chromatography chromatography Subject Category: Techniques, Methodologies and Equipment see more details, fatty acids fatty acids Subject Category: Chemicals and Chemical Groups see more details, methodology methodology Subject Category: Techniques, Methodologies and Cited by: 5.

Qualitative and Quantitative Determination of The Fatty Acids by Gas‐Liquid Chromatography. James. National Institute for Medical Research, London Book Series: Methods of Biochemical Analysis.

About. PDF. Tools. Request permission; Separation of Fatty Acids. Quantitative Determination of the Fatty by: Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor Agonist Haruya TAKAHASHI,1 Hideyuki SUZUKI,2 Kunihiro SUDA,2 Yota YAMAZAKI,1 Akihiro TAKINO, 1Young-Il KIM, Tsuyoshi GOTO,1;3 Yoko IIJIMA,2 Koh AOKI,2.

The qualitative and quantitative profiling of fatty acids in human blood is a useful tool in disease prevention and health care, two concepts that are intimately related.

Advances In The Synthesis Of Glycerides Of Fatty Acids. Chemical Reviews58 (5), DOI: /cra J. Labarrere, J. Chipault, and W. Lundberg. Cholesteryl Esters of Long-Chain Fatty Acids.

Infrared Spectra and Separation by Paper by:   Abstract. Long chain fatty acids (LCFAs) are present in various natural samples and are easily detectable using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) in negative ion mode.

Chemical composition characteristics, including the content of fatty acids, which could be carried out using the gas chromatography technique, are an important element connecting the chemical industry with research and allow the optimization of scCO 2 extraction processes.

The whole procedure allows better quality control of the final product. 1. Introduction. Suet oil (SO), a fatty oil obtained from the domestic goat Quantitative paper chromatography of ¹⁴C-labelled long-chain fatty acids book hircus Linnaeus) or sheep (Ovis aries Linnaeus), has been used in the food industry [] and the medicine industry [].SO is rich in unsaturated and saturated fatty acids (FAs) [], which are involved in a number of important physiological provide energy to the cell and act as substrates in the.

This book outlines the basic understanding of various chromatographic techniques like paper chromatography, thin layer chromatography and column chromatography. A gas chromatography–flame ionization detection (GC–FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material.

Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m× mm i.d.) with a total run. When compared with wet blood, the fatty acid determinations from the dried blood spots were largely similar although there were some minor differences in low abundant fatty acids.

Quantitative fatty acid determinations of dried blood spots are possible and should be reported along with relative percentage data to improve interpretation in the.

Slover HT, Lanza E. Quantitative analysis of food fatty acids by capillary gas chromatography. J Am Oil Chem Soc. ; – doi: /BF [ Cross Ref ]. Quantitative analysis of food fatty acids by capillary gas chromatography This paper discusses the use of wall‐coated glass capillary columns in a semiautomated system for the determination of food fatty acids.

Hanna Kontkanen, Kristiina Kruus, Screening of microbes for lipases specific for saturated medium and long-chain fatty acids. Abstract The development of a new class of derivatives, the alkyldimethylaminoethyl ester iodides, for the analysis of fatty acids by electrospray ionization tandem mass spectrometry is described.

Long-chain acyl-CoA esters (LCACoAs) are activated lipid species that represent key substrates in lipid metabolism. The relationship between lipid metabolism disorders and type 2 diabetes has attracted much attention to this class of metabolites. This paper presents a highly sensitive and robust on-line LC/MS2 procedure for quantitative determination of LCACoAs from rat liver.

A fast. High Performance Liquid Chromatography (HPLC) There are few excellent original papers and reviews until today which are focused on usage of liquid column chromatography for the analysis of fatty acids (saturated and unsaturated) and also their related substances in biological, food, and drugs samples [18, 93–96].In these papers principles of HPLC including the sample preparation, mobile.

For complete analysis of natural mixtures of fatty acids it is advisable at the present time to combine paper chromatography with some other separation method. Distillation supplements best but requires gram quantities of material while paper chromatography is applicable on gamma or milligram scale.

On a microcolumn of neutral alumina, long-chain fatty acids are quantitatively retained. Neutral lipids including cholesteryl esters, triglycerides, diglycerides and fatty acid methyl esters, as well as cholesterol, are quantitatively recovered in the effluent without hydrolysis of ester.

A note on the preparation of cholesteryl esters of long-chain fatty acids. Journal of the American Oil Chemists Society37 (12), DOI: /BF Č. Michalec, J. Strašek. A new paper chromatographic technique for the separation of cholesterol and cholesteryl esters. A procedure is described for the rapid preparation of phenacyl and naphthacyl derivatives of fatty acids.

These derivatives of saturated, monounsaturated, and polyunsaturated fatty acids were analyzed by high-performance liquid chromatography (HPLC) on a C 18 reversed-phase column at nanogram sensitivity.

Standard mixtures of fatty acids gave quantitative mole percentages. The short-chain fatty acids, propionic to pelargonic, have been analyzed by gas liquid chromatography, as their butyl, phenacyl, and decyl esters to overcome losses due to their volatility.

The three methods are compared and the decyl ester procedure offers the most advantages.Quantitative analysis of food fatty acids by capillary gas chromatography. H. T. Slover The superior efficiency of capillary columns is desirable for the gas chromatographic analysis of complex mixtures of fatty acids, but there have been some reservations regarding quantitation and reproducibility.

This paper discusses the use of wall.In this study, a gas chromatography‐mass spectrometry method was developed and validated for the analysis of short‐chain fatty acids in faecal samples. These volatile compounds were extracted with ethyl acetate and 4‐methyl valeric acid was used as an internal standard.